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murine pituitary corticotrophin tumor cell line att  (ATCC)


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    ATCC murine pituitary corticotrophin tumor cell line att
    Murine Pituitary Corticotrophin Tumor Cell Line Att, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 350 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pituitary+tumor+cell+line/AtT-20/10__1186_slash_s12575___026___00359___9-61-12-23
    Average 95 stars, based on 350 article reviews
    murine pituitary corticotrophin tumor cell line att - by Bioz Stars, 2026-11
    95/100 stars

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    other:

    Article Title: Protein kinase C delta mediates Pasireotide effects in an ACTH-secreting pituitary tumor cell line
    Article Snippet: The mouse ACTH-secreting pituitary tumor cell line, AtT-20/D16v-F2 (ATCC CRL-1795, American Type Culture Collection, Manassas, Virginia, USA), was maintained at 37 °C, 5% CO 2 in DMEM high glucose (Invitrogen, Carlsbad, California, USA) supplemented with 10% horse serum (LGC Standards, Milan, Italy) and antibiotic/antimycotic (EuroClone, Milan, Italy).

    Article Title: Protein kinase C delta mediates Pasireotide effects in an ACTH-secreting pituitary tumor cell line.
    Article Snippet: The mouse ACTH-secreting pituitary tumor cell line, AtT20/D16v-F2 (ATCC CRL-1795, American Type Culture Collection, Manassas, Virginia, USA), was maintained at 37 °C, 5% CO2 in DMEM high glucose (Invitrogen, Carlsbad, California, USA) supplemented with 10% horse serum (LGC Standards, Milan, Italy) and antibiotic/antimycotic (EuroClone, Milan, Italy).

    Cell Culture:

    Article Title: TNF-α-Induced VEGF and MMP-9 Expression Promotes Hemorrhagic Transformation in Pituitary Adenomas
    Article Snippet: .. MMQ, a rat prolactin-secreting pituitary tumor cell line, was purchased from ATCC (American Type Culture Collection, Manassas, VA) and routinely cultured in F-12 medium supplemented with 2.5% fetal bovine serum and 15% equine serum at 37 °C in a humidified air atmosphere containing 5% carbon dioxide in T-75 flasks. .. MMQ cells were stimulated with 20 ng/mL TNF-α (Abcam) or saline for 24 h, and then were analyzed for possible effects of TNF-α on VEGF and MMP-9 expression.



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    murine pituitary corticotrophin tumor cell line att  (ATCC)
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    a CCK-8 cell viability assay of GH3, <t>AtT-20,</t> and TtT/GF cells treated with OPN or PBS ( n = 5 biological replicates). Data are presented as mean values +/- SD. b Representative images of the wound healing assay, and ( c ) quantitative analysis of TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. d Transwell invasion assay and ( e ) quantitative analysis of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. f Immunofluorescence images of PitNET markers (SYN, SF1, FSH) in PitNET organoids from two independent patients ( n = 3 biological replicates). g Transwell invasion assay and ( h ) quantitative analysis of primary pituitary tumor cells cultured from two independent patients, treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. i Bright-field images illustrating representative phenotypes of three independently derived PitNET organoids from each of the two patients treated with OPN or PBS. Arrows indicate cell protrusions in PitNET organoids. j CellTiter-Glo luminescent cell viability assay of PitNET organoids treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. k Immunofluorescence images of Ki67 in PitNET organoids from two patients treated with OPN or PBS ( n = 3 biological replicates). For all panels, scale bar, 100 µm. Two-sided unpaired t -test was performed for ( a − j ). Data are presented as mean values +/- SD for ( a − j ). Source data are provided as a Source Data file for ( a − j ).
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    a CCK-8 cell viability assay of GH3, <t>AtT-20,</t> and TtT/GF cells treated with OPN or PBS ( n = 5 biological replicates). Data are presented as mean values +/- SD. b Representative images of the wound healing assay, and ( c ) quantitative analysis of TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. d Transwell invasion assay and ( e ) quantitative analysis of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. f Immunofluorescence images of PitNET markers (SYN, SF1, FSH) in PitNET organoids from two independent patients ( n = 3 biological replicates). g Transwell invasion assay and ( h ) quantitative analysis of primary pituitary tumor cells cultured from two independent patients, treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. i Bright-field images illustrating representative phenotypes of three independently derived PitNET organoids from each of the two patients treated with OPN or PBS. Arrows indicate cell protrusions in PitNET organoids. j CellTiter-Glo luminescent cell viability assay of PitNET organoids treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. k Immunofluorescence images of Ki67 in PitNET organoids from two patients treated with OPN or PBS ( n = 3 biological replicates). For all panels, scale bar, 100 µm. Two-sided unpaired t -test was performed for ( a − j ). Data are presented as mean values +/- SD for ( a − j ). Source data are provided as a Source Data file for ( a − j ).
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    a CCK-8 cell viability assay of GH3, <t>AtT-20,</t> and TtT/GF cells treated with OPN or PBS ( n = 5 biological replicates). Data are presented as mean values +/- SD. b Representative images of the wound healing assay, and ( c ) quantitative analysis of TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. d Transwell invasion assay and ( e ) quantitative analysis of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. f Immunofluorescence images of PitNET markers (SYN, SF1, FSH) in PitNET organoids from two independent patients ( n = 3 biological replicates). g Transwell invasion assay and ( h ) quantitative analysis of primary pituitary tumor cells cultured from two independent patients, treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. i Bright-field images illustrating representative phenotypes of three independently derived PitNET organoids from each of the two patients treated with OPN or PBS. Arrows indicate cell protrusions in PitNET organoids. j CellTiter-Glo luminescent cell viability assay of PitNET organoids treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. k Immunofluorescence images of Ki67 in PitNET organoids from two patients treated with OPN or PBS ( n = 3 biological replicates). For all panels, scale bar, 100 µm. Two-sided unpaired t -test was performed for ( a − j ). Data are presented as mean values +/- SD for ( a − j ). Source data are provided as a Source Data file for ( a − j ).
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    a CCK-8 cell viability assay of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 5 biological replicates). Data are presented as mean values +/- SD. b Representative images of the wound healing assay, and ( c ) quantitative analysis of TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. d Transwell invasion assay and ( e ) quantitative analysis of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. f Immunofluorescence images of PitNET markers (SYN, SF1, FSH) in PitNET organoids from two independent patients ( n = 3 biological replicates). g Transwell invasion assay and ( h ) quantitative analysis of primary pituitary tumor cells cultured from two independent patients, treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. i Bright-field images illustrating representative phenotypes of three independently derived PitNET organoids from each of the two patients treated with OPN or PBS. Arrows indicate cell protrusions in PitNET organoids. j CellTiter-Glo luminescent cell viability assay of PitNET organoids treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. k Immunofluorescence images of Ki67 in PitNET organoids from two patients treated with OPN or PBS ( n = 3 biological replicates). For all panels, scale bar, 100 µm. Two-sided unpaired t -test was performed for ( a − j ). Data are presented as mean values +/- SD for ( a − j ). Source data are provided as a Source Data file for ( a − j ).

    Journal: Nature Communications

    Article Title: Single-cell and spatial transcriptome analyses reveal tumor heterogeneity and immune remodeling involved in pituitary neuroendocrine tumor progression

    doi: 10.1038/s41467-025-60028-5

    Figure Lengend Snippet: a CCK-8 cell viability assay of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 5 biological replicates). Data are presented as mean values +/- SD. b Representative images of the wound healing assay, and ( c ) quantitative analysis of TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. d Transwell invasion assay and ( e ) quantitative analysis of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. f Immunofluorescence images of PitNET markers (SYN, SF1, FSH) in PitNET organoids from two independent patients ( n = 3 biological replicates). g Transwell invasion assay and ( h ) quantitative analysis of primary pituitary tumor cells cultured from two independent patients, treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. i Bright-field images illustrating representative phenotypes of three independently derived PitNET organoids from each of the two patients treated with OPN or PBS. Arrows indicate cell protrusions in PitNET organoids. j CellTiter-Glo luminescent cell viability assay of PitNET organoids treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. k Immunofluorescence images of Ki67 in PitNET organoids from two patients treated with OPN or PBS ( n = 3 biological replicates). For all panels, scale bar, 100 µm. Two-sided unpaired t -test was performed for ( a − j ). Data are presented as mean values +/- SD for ( a − j ). Source data are provided as a Source Data file for ( a − j ).

    Article Snippet: The pituitary tumor cell line TtT/GF(Procell, CL-0561) is cultured in DMEM/F12 medium supplemented with 10% horse serum, 2.5% FBS, 2 ng/ml basic fibroblast growth factor (bFGF), and 1% penicillin-streptomycin (Procell, CM-0561).

    Techniques: CCK-8 Assay, Viability Assay, Wound Healing Assay, Transwell Invasion Assay, Immunofluorescence, Cell Culture, Derivative Assay, Cell Viability Assay

    a Western blot analysis of ITGB1 expression in GH3, AtT-20, and TtT/GF cell lines. b Semi-quantitative analysis of ITGB1 in GH3, AtT-20, and TtT/GF cell lines ( n = 3 biological replicates). c CCK-8 cell viability assay of GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). d Transwell invasion assay of GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). e Quantitative analysis of Transwell invasion assay in GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). Scale bar, 100 µm. Two-sided unpaired t -test was performed for ( b − e ). Data are presented as mean values +/- SD for ( b − e ). Source data are provided as a Source Data file for ( a − e ).

    Journal: Nature Communications

    Article Title: Single-cell and spatial transcriptome analyses reveal tumor heterogeneity and immune remodeling involved in pituitary neuroendocrine tumor progression

    doi: 10.1038/s41467-025-60028-5

    Figure Lengend Snippet: a Western blot analysis of ITGB1 expression in GH3, AtT-20, and TtT/GF cell lines. b Semi-quantitative analysis of ITGB1 in GH3, AtT-20, and TtT/GF cell lines ( n = 3 biological replicates). c CCK-8 cell viability assay of GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). d Transwell invasion assay of GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). e Quantitative analysis of Transwell invasion assay in GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). Scale bar, 100 µm. Two-sided unpaired t -test was performed for ( b − e ). Data are presented as mean values +/- SD for ( b − e ). Source data are provided as a Source Data file for ( a − e ).

    Article Snippet: The pituitary tumor cell line TtT/GF(Procell, CL-0561) is cultured in DMEM/F12 medium supplemented with 10% horse serum, 2.5% FBS, 2 ng/ml basic fibroblast growth factor (bFGF), and 1% penicillin-streptomycin (Procell, CM-0561).

    Techniques: Western Blot, Expressing, CCK-8 Assay, Viability Assay, Transwell Invasion Assay

    a CCK-8 cell viability assay of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 5 biological replicates). Data are presented as mean values +/- SD. b Representative images of the wound healing assay, and ( c ) quantitative analysis of TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. d Transwell invasion assay and ( e ) quantitative analysis of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. f Immunofluorescence images of PitNET markers (SYN, SF1, FSH) in PitNET organoids from two independent patients ( n = 3 biological replicates). g Transwell invasion assay and ( h ) quantitative analysis of primary pituitary tumor cells cultured from two independent patients, treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. i Bright-field images illustrating representative phenotypes of three independently derived PitNET organoids from each of the two patients treated with OPN or PBS. Arrows indicate cell protrusions in PitNET organoids. j CellTiter-Glo luminescent cell viability assay of PitNET organoids treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. k Immunofluorescence images of Ki67 in PitNET organoids from two patients treated with OPN or PBS ( n = 3 biological replicates). For all panels, scale bar, 100 µm. Two-sided unpaired t -test was performed for ( a − j ). Data are presented as mean values +/- SD for ( a − j ). Source data are provided as a Source Data file for ( a − j ).

    Journal: Nature Communications

    Article Title: Single-cell and spatial transcriptome analyses reveal tumor heterogeneity and immune remodeling involved in pituitary neuroendocrine tumor progression

    doi: 10.1038/s41467-025-60028-5

    Figure Lengend Snippet: a CCK-8 cell viability assay of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 5 biological replicates). Data are presented as mean values +/- SD. b Representative images of the wound healing assay, and ( c ) quantitative analysis of TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. d Transwell invasion assay and ( e ) quantitative analysis of GH3, AtT-20, and TtT/GF cells treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. f Immunofluorescence images of PitNET markers (SYN, SF1, FSH) in PitNET organoids from two independent patients ( n = 3 biological replicates). g Transwell invasion assay and ( h ) quantitative analysis of primary pituitary tumor cells cultured from two independent patients, treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. i Bright-field images illustrating representative phenotypes of three independently derived PitNET organoids from each of the two patients treated with OPN or PBS. Arrows indicate cell protrusions in PitNET organoids. j CellTiter-Glo luminescent cell viability assay of PitNET organoids treated with OPN or PBS ( n = 3 biological replicates). Data are presented as mean values +/- SD. k Immunofluorescence images of Ki67 in PitNET organoids from two patients treated with OPN or PBS ( n = 3 biological replicates). For all panels, scale bar, 100 µm. Two-sided unpaired t -test was performed for ( a − j ). Data are presented as mean values +/- SD for ( a − j ). Source data are provided as a Source Data file for ( a − j ).

    Article Snippet: Pituitary tumor cell lines AtT-20(ATCC, CRL-1795), GH3(ATCC, CCL-82.1) were cultured in DMEM (ThermoFisher, 11995065) medium supplemented with 10% fetal bovine serum (FBS) (ThermoFisher, 10270106) and 1% penicillin-streptomycin (ThermoFisher, 15070063) under standard conditions of 37 °C in a humidified incubator with 5% CO 2 .

    Techniques: CCK-8 Assay, Viability Assay, Wound Healing Assay, Transwell Invasion Assay, Immunofluorescence, Cell Culture, Derivative Assay, Cell Viability Assay

    a Western blot analysis of ITGB1 expression in GH3, AtT-20, and TtT/GF cell lines. b Semi-quantitative analysis of ITGB1 in GH3, AtT-20, and TtT/GF cell lines ( n = 3 biological replicates). c CCK-8 cell viability assay of GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). d Transwell invasion assay of GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). e Quantitative analysis of Transwell invasion assay in GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). Scale bar, 100 µm. Two-sided unpaired t -test was performed for ( b − e ). Data are presented as mean values +/- SD for ( b − e ). Source data are provided as a Source Data file for ( a − e ).

    Journal: Nature Communications

    Article Title: Single-cell and spatial transcriptome analyses reveal tumor heterogeneity and immune remodeling involved in pituitary neuroendocrine tumor progression

    doi: 10.1038/s41467-025-60028-5

    Figure Lengend Snippet: a Western blot analysis of ITGB1 expression in GH3, AtT-20, and TtT/GF cell lines. b Semi-quantitative analysis of ITGB1 in GH3, AtT-20, and TtT/GF cell lines ( n = 3 biological replicates). c CCK-8 cell viability assay of GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). d Transwell invasion assay of GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). e Quantitative analysis of Transwell invasion assay in GH3, AtT-20, and TtT/GF cell lines under specified treatments ( n = 3 biological replicates). Scale bar, 100 µm. Two-sided unpaired t -test was performed for ( b − e ). Data are presented as mean values +/- SD for ( b − e ). Source data are provided as a Source Data file for ( a − e ).

    Article Snippet: Pituitary tumor cell lines AtT-20(ATCC, CRL-1795), GH3(ATCC, CCL-82.1) were cultured in DMEM (ThermoFisher, 11995065) medium supplemented with 10% fetal bovine serum (FBS) (ThermoFisher, 10270106) and 1% penicillin-streptomycin (ThermoFisher, 15070063) under standard conditions of 37 °C in a humidified incubator with 5% CO 2 .

    Techniques: Western Blot, Expressing, CCK-8 Assay, Viability Assay, Transwell Invasion Assay